Transgenic food safety is a high-profile public health issue in worldwide, especially transgenic soybean (Glycine max L.) oil. To rapidly and effectively detect transgenic components of soybean oil, in the present study, we isolated DNA from transgenic soybean oil by modified method, and employed the multiplex PCR method to identify targeted genes, including CaMV35S promoter, Nos terminator, NPTII, CP4-EPSPS and endogenous gene Lectin. The research aims to build a method which is accurate, rapid and reliable for detection of genetically modified soybeans oil. The targeted gene including DNA was successfully established by the improved method, and then amplified by PCR. Five genes are simultaneously specifically detected. Commercial soybean, genetically modified soy bean and oil were detected with the Multiplex PCR. The improved method of DNA extraction was rapid and accurate to extract high quality total DNA which was amplified by PCR. The method could eliminate the PCR inhibitor. A way of detecting the genetically modified soybean and Oil was set up in this study.
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机译：转基因食品安全是在全球范围内的高调公共卫生问题，特别是转基因大豆（Glycine Max L.）油。为了快速和有效地检测大豆油的转基因组分，在本研究中，通过改性方法从转基因大豆油中分离DNA，采用多重PCR方法来鉴定靶向基因，包括Camv35s启动子，NOS终止子，NPTII，CP4-EPSPS。和内源性基因凝集素。该研究旨在建立一种方法，可用于检测转基因大豆油的准确，快速可靠。通过改进的方法成功建立包括DNA的靶向基因，然后通过PCR扩增。同时发现五个基因。用多重PCR检测商业大豆，遗传改性大豆和油。改进的DNA提取方法快速准确，以提取通过PCR扩增的高质量总DNA。该方法可以消除PCR抑制剂。在本研究中建立了一种检测遗传改性大豆和油的方法。
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