The application of near-infrared spectra micro-image in the imaging analysis of biology samples

摘要

In this research,suitable imaging methods were used for acquiring single compound images ofbiology samples of chicken pectorales tissue section,tobacco dry leaf,fresh leaf and plantglandular hair,respectively.The adverse effects caused by the high water content and thethermal effect of near infrared(NIR)light were effectively solved during the experiment procedures and the data procesing.PCA algorithm was applied to the NIR micro-image of chickenpectorales tissue.Comparing the loading vector of PC3 with the NIR spectrum of dry albumen,the information of PC3 was confimmed to be provided mainly by protein,i.e.,the 3rd score imagerepresents the distribution trend of protein mainly.PCA algorithm was applied to the NIR micro-image of tobacco dry leaf.The information of PC2 was confimed to be provided by carbohydrateindluding starch mainly.Compared to the 2nd score image of tobacco dry leaf,the comparedcorelation image with the reference spectrum of starch had the same distribution trend as the 2nd score image.The comparative correla tion images with the reference spectra of protein,glucose,fructose and the total plant alkaloid were acquired to confirm the distribution trend ofthese compounds in tobacco dry leaf respectively.Comparative correlation images of fresh leafwith the reference spectra of protein,starch,fructose,ghucose and water were acquired to confim the distribution trend of these compounds in fresh leaf.Chemimap imaging of plant glandularhair was acquired to show the tubular structure clearly.