Novel in vivo Evaluation of the Moisturizing Effect of A Lotion by Using Raman Microspectroscopy

摘要

Skin moisturization can be measured in vivo by Corneometer?, giving information about the hydration level of the skin. However, this approach cannot reveal the inner changes of lipid organization involved in the integrity of the skin barrier. Raman microspectroscopy is an in vivo and non-invasive method that assesses the lateral organization of lipids from the stratum corneum by measuring the νasymCH2/νsymCH2 ratio. In the present study, the aim is to evaluate the moisturizing effect of two skin care products (i.e. LOTION 1:GF deep-nourishing & skin-sturdy facial emulsion; LOTION 2: placebo formula) by using the Corneometer? and Raman microspectroscopy. Results demonstrates a significant improvement of lipids organization and thus a reinforcement of the barrier function associated with an increase of the hydration level with LOTION 1 compared to LOTION 2.