The associated production of Z boson and a pair of new quarks at the Large Hadron Collider (LHC) is studied. The cross sections for both sequential fermions and vector-like fermions are presented. It is found that for sequential fermions the cross sections can reach 1 ～10~2 fb for heavy quark mass m_Q from 1000 GeV to 200 GeV. For vector-like quarks, the cross sections are suppressed by mixing parameter sin θ_L. Focusing on process pp →b′b′, we investigate the possibility of detecting the 61+2j signal. For a b′ with light mass and a large branching ratio of b′→ bZ, it is found that only several signal events (parton level) can be produced with 1000 fb^(-1) integrated luminosity. Although the signal events are rare, all the final states are produced centrally and multi lepton final states are clear at hadron collider, which could be easily detected.
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