Effect of DNA damage caused by cryopreservation of spermatozoa using a modified Single cell gell electrophoresis in the freshwater catfish Pangasianodon hypophthalmus (Fowler, 1936)

摘要

Objective: To detect the integrity of sperm DNA in the catfish Pangasianodon hypophthalmus scirnygolper-ecseelrlv egedl weliethc tHroapnhkosr ebsaisl ainnc oerdd esra ltto stoelsutt ihoonw, 5s%pe-r3m0% c rdyiomperethseyrlv aactieotna mafifdeec t(eDdM nAu) culseianrg D tNhAe stability. Methods: Electrophoresis was conducted for 60 min at 130 mA and 15 V. The comet images awnedre p aenrcaelynzteadge u DsiNnAg isno ftthwea rtaei lC womereet Socbotraein 1e.d5,. aTnhde np tahrea mceotmerest rsautceh a ansd cdoammeatg lee ncgotehf,f ictaieiln lte wngetrhe calculated. Results: There were no significant differences in motility, comet rate and damage coefficient sbpeetwrmee cnr yforepsrhe sseprveermd wainthd 2c5r%yo apnreds 3e0r%ve dD MsAp ehramd sltoowreerd mino t5il%it,y 1, 0h%ig, h1e5r% c oamnde t2 l0e%n gDthM Aa,n dw hdialme athgee coefficients than those of fresh sperm. Conclusions: There was a positive correlation between comet rate of cryopreserved sperm and the concentration of DMA.The toxicity of cryoprotectant is the main factor for DNA damage in cryopreserved sperm.