Isolation and characterization of endosulfan-degrading bacteria from contaminated agriculture soils

摘要

Objective: To isolate and characterize endosulfan-degrading bacteria from Kerman pistachio orchards. Methods: Endosulfan-degrading bacteria were enriched in Bushnell-Hass medium. Identification and sequencing of prevalent degrading strains was performed by usingPCR based on amplifying16S rDNA. Results: The results showed that the soils of pistachio orchards have some degrading bacteria that are suitable for elimination of endosulfan from soils and the environment. Four endosulfan-degrading bacteria strains belong toAchromobacter xylosoxidans (strain EN3),Pseudomonas azotoformans (strain EN4),Pseudomonas brassicacearum (strain EN7) andPseudomonas thivervalensis (strain EN8), respectively. The best degrading strain (EN7), up to 100 mg/L, illustrated a good growth, whereas the growth was reduced in concentration higher than 100 mg/L. The results of gas chromatography confirmed the decomposition of organic pesticide by degrading-bacteria. Conclusions: By using these strains and other biological reclamation methods we can eliminate bio-environmental problems.