Research of expression and correlation of ADAM8 and EGFR in the osteosarcoma tissue

摘要

Objective:To lay a foundation for the research on the targeted therapy of osteosarcoma or the regulatory mechanism of the occurrence and development of osteosarcoma by studying the expression of a disintegrin and metalloproteinase domain-containing protein 8(ADAM8)and epithelial growth factor receptor(EGFR)in osteosarcoma.Methods:Osteosarcoma tissues,osteochondroma tissues,osteoarthritis tissues and normal bone tissues were selected and included in this research.By use of tissue chips and immunohistochemistry,the expression levels of ADAM8 and EGFR in the previously-mentioned tissues were observed and compared.Spearman’s rank correlation test was used to analyze the correlation of ADAM8 and EGFR and their relationship with clinicopathological factors in these cases.Results:The positive expression levels of ADAM8 in osteosarcoma tissues,osteochondroma tissues,osteoarthritis tissues and normal bone tissues were 80.95%,36.36%,62.86%and 24.14%respectively,the difference was of statistical significance(p<.05);the positive expression levels of EGFR in osteosarcoma tissues,osteochondroma tissues,osteoarthritis tissues and normal bone tissues were 66.67%,9.09%,40.00%and 4.76%respectively,the difference was of statistical significance(p<.05);Spearman’s rank correlation test showed that ADAM8 was positively correlated with EGFR(r=.379,p<.05).Conclusions:Both ADAM8 and EGFR have a much higher expression in osteosarcoma tissues,and the combined detection of two indexes can be considered as a new method in the clinical diagnosis of osteosarcoma.