Effects of benner pury on the function of endothelial progenitor cells in vitro from patients with hypertension in a time-dependent manner

摘要

Objective:To investigate the effect of Benner Pury on Endothelial progenitor cells(EPCs)proliferation,migration,adhesion,apoptosis and oxidative stress in vitro from patients with hypertension and its time dependence.Methods:The patients who were diagnosed as hypertension grade 1 according to the standard“Guidelines for Prevention and Treatment of Hypertension”were enrolled.Mononuclear cells were isolated by density gradient centrifugation and stained with fluorescence chemical acetylated low density lipoprotein marked with DiI(acLDL-DiI)and fluorescein isothiocyanate(FITC)-lectin.Double staining positive cells were considered as the differentiation of EPCs.The control group included healthy subjects matched with study group in age,gender.EPCs cultivated for 5 days were used for study.Cells harvested at different set times under the stimulation of Benner Pury,and the EPCs proliferation,migration,adhesion ability,apoptosis and oxidative stress indicators were detected respectively.Results:(1)Compared with the control group,peripheral blood EPCs proliferation,migration and adhesion ability were ob-viously decreased in the hypertension group(p<.01),EPCs apoptosis rate and oxidative stress response were significantly increased(p<.01).(2)Benner Pury significantly increased the EPCs proliferation,migration,adhesion ability and improved apoptosis and oxidative stress reaction in a time-dependent manner.Conclusions:Benner Pury can improve the EPCs proliferation,migration,adhesion,apoptosis and oxidative stress in patients with hypertension in a time-dependent manner.