Effects of benner pury on the function of endothelial progenitor cells in vitro from patients with hypertension in a time-dependent manner

摘要

Objective: To investigate the effect of Benner Pury on Endothelial progenitor cells (EPCs) proliferation, migration, adhesion, apoptosis and oxidative stress in vitro from patients with hypertension and its time dependence. Methods: The patients who were diagnosed as hypertension grade 1 according to the standard "Guidelines for Prevention and Treatment of Hypertension" were enrolled. Mononuclear cells were isolated by density gradient centrifugation and stained with fluorescence chemical acetylated low density lipoprotein marked with DiI (acLDL-DiI) and fluorescein isothiocyanate (FITC)-lectin. Double staining positive cells were considered as the differentiation of EPCs. The control group included healthy subjects matched with study group in age, gender. EPCs cultivated for 5 days were used for study. Cells harvested at different set times under the stimulation of Benner Pury, and the EPCs proliferation, migration, adhesion ability, apoptosis and oxidative stress indicators were detected respectively. Results: (1) Compared with the control group, peripheral blood EPCs proliferation, migration and adhesion ability were ob-viously decreased in the hypertension group (p < .01), EPCs apoptosis rate and oxidative stress response were significantly increased (p < .01). (2) Benner Pury significantly increased the EPCs proliferation, migration, adhesion ability and improved apoptosis and oxidative stress reaction in a time-dependent manner. Conclusions: Benner Pury can improve the EPCs proliferation, migration, adhesion, apoptosis and oxidative stress in patients with hypertension in a time-dependent manner.