载脂蛋白J及补体C3在大鼠脑出血模型中表达的实验研究

摘要

目的 探索载脂蛋白J(ApoJ)及补体C3在实验性大鼠脑出血(ICH)损伤中的作用机制.方法 将雌雄各半Sprauge-Dawley(SD)大鼠150只随机分为正常对照组、假手术组及ICH组.通过脑立体定向仪建立大鼠ICH模型,于建模后3、6、12 h以及1、3、5、7 d 7个时间点处死大鼠.用干湿质量法测量脑组织含水量;RT-PCR检测ApoJ mRNA及补体C3 mRNA的表达水平.结果 ICH组ApoJ、补体C3 mRNA的表达水高于假手术组和正常对照组(P＜0.05),补体C3、ApoJ mRNA的表达分别于ICH后3、6 h开始增强;ICH组ApoJ、补体C3 mRNA表达水平、脑组织含水量呈正相关(R=0.758,P＜0.05;R=0.702,P＜0.05),ApoJ mRNA与补体C3 mRNA表达水平呈正相关(R=0.908,P＜0.05).结论 补体系统参与了大鼠ICH后脑水肿的形成及神经元的损伤;ApoJ可能通过抑制补体激活而发挥ICH后机体内源性应激保护作用.%To explore mechanisms of apolipoprotein J(ApoJ) and complement component 3 ( C3) in experimental injury of intracerebral hemorrhage(ICH) in rats.Methods 150 Sprauge-Dawley rats, half male and half female, were randomly divided into 3 groups: normal control,sham operation and ICH group.Rats ICH models were established through brain stereotactic instrument.Rats were sacrificed at seven time points after modeling: 3 h,6 h,12 h,1 d,3 d,5 d and 7 d.Dry and wet quality method was employed to measure brain water content and expression level of ApoJ and complement C3 mRNA were detected by RT-PCR.Results Both expression level of ApoJ and complement C3 mRNA in brain tissue in ICH group were higher than that in sham operation and normal control group(P＜0.05) and the expression of ApoJ and complement C3 mRNA started to increase in 3 hours and 6 hours after ICH , respectively;ln ICH group,levels of ApoJ and complement C3 mRNA expression had positive correlation with brain water content(R = 0.758,P＜0.05 ;R = 0.702 , P＜0.05) , and there was also a positive correlation between expression level of ApoJ and complement C3 mRNA(R=0.908,P＜0.05).Conclusion Complement system may participates in formation of brain edema and the injury of neuron after ICH in rats.ApoJ probably play an endogenous stress protective role after ICH via inhibition of complement activation.