预防腹腔镜管腔器械产生生物膜的清洗方法探讨

摘要

Objective To study the washing methods for the piping instruments of laparoscope and prevention form biological membrane .Methods 300 pieces of the piping instruments of laparoscope select from the center of sterilization supply were random classified into 3 groups(100 pieces in each group) according to washing methods :washing by hand(group A) ,full-automation wash-ing machine(group B) and Tri-band pressurized ultrasonic washing machine(group C) .Comparing the cleanliness and ATP detec-tion fluorescence value on different methods .Results Cleanliness of group A was 85% ,ATP fluorescence detection rate was 27% ;Cleanliness of group B was 82% ,ATP fluorescence detection positive rate was 31% ;Cleanliness of group C was 99% ,ATP fluores-cence detection positive rate was 5% .The test data of group C was significantly compare with the data of group A and B . Conclusion The washing method of using tri-band pressurized ultrasonic washing machine can effectively improve laparoscopic tube cavity equipment cleaning effect and prevent form biological membrane .%目的：探讨腹腔镜管腔器械的清洗方法，预防腹腔镜管腔器械生物膜的产生。方法选择消毒供应中心回收的污染腹腔镜管腔手术器械300件，根据清洗方法分成A组（手工清洗法）、B组（全自动清洗机清洗法）、C组（三频超声波合并加压清洗法），每组100件。比较不同方法清洗后器械的洁净度和三磷腺苷（ATP）检测荧光值。结果 A组洁净度为85％，ATP荧光阳性率27％；B组洁净度为82％，A T P荧光阳性率为31％；C组洁净度为99％，A T P荧光阳性率为5％。C组各测试结果与A、B组比较，差异有统计学意义（P＜0．05）。结论用三频超声波合并加压法清洗腹腔镜管腔器械，能有效提高清洗效果，可预防管腔器械生物膜的产生。