间充质干细胞移植促进放射性肠损伤肠绒毛的结构修复

摘要

目的 研究人脐带间充质干细胞(hMSCs)对放射性肠损伤小鼠存活率的影响及其对肠道损伤修复的作用.方法 选取成年C57BL/6J小鼠,分为空白对照组,腹部照射+PBS组(IR+PBS)和腹部照射+hMSCs移植治疗组(IR+hMSCs).13 Gyγ射线腹部照射造成肠损伤模型,检测30 d存活率,另外分别于照射后的第3.5天和第5.0天,HE染色观察各组十二指肠、空肠和回肠的绒毛结构改变和肠隐窝的数量变化.结果 相较于IR+PBS组的小鼠,IR+hMSCs组的小鼠存活率明显提高(P＜0.05).与空白对照组比较,13 Gyγ射线腹部照射后第3.5天,IR+PBS组的十二指肠、空、回肠绒毛均发生断裂、长度变短且数目稀少,而IR+hMSCs组肠损伤减轻.至照射后第5.0天,IR+PBS组小肠结构完整性有所改善,但达不到IR+ hMSCs组的水平(P＜0.01).照射后的第3.5天和第5.0天,IR+ hMSCs组小鼠肠隐窝的数目明显多于IR+PBS组,但低于空白对照组(均P＜0.01).结论 MSCs可以提高放射性肠损伤小鼠存活率并且对损伤肠道具有促修复作用,其有望用于放射性肠损伤的治疗.%Objective To study the effect of human umbilical cord mesenchymal stem cells (hMSCs) on the survival rate of mice with radiation-induced intestinal injury and its effect on the repair of intestinal damage.Methods Adult C57BL/6J mice were selected and divided into control group and abdomen irradiation + PBS(IR + PBS) group and abdominal irradiation+hMSCs transplantation treatment (IR+hMSCs)group.Intestinal injury model was induced by abdominal irradiation at 13 Gy of T ray and the 30-day survival rate was recorded.On the 3.5th and 5.0th day after irradiation,HE staining was used to observe changes in the villus structure and thc number of intestinal crypts in each segment of the small intestine (duodenum,jejunum,and ileum).Results The survival rate of IR+hMSCs group was significantly higher than that of IR+PBS group (P＜0.05).On the 3.5th day after 13 Gy of abdomen irradiation,the duodenum,cavities and ileum villus in IR+PBS group were all fractured,the length was shortened,and the numbers were sparse when compared with the control group,while the hMSCs reduced the intestinal damage.On the 5.0th day after irradiation,the structural integrity of the small intestine in IR+PBS group was repaired slightly,but it still more serious than that of IR+hMSCs group (P＜0.01).The amount of intestine crypts were significantly higher in IR+hMSCs group than that of IR+PBS group,but were significantly lower than that of control group (P＜0.01) at 3.5th day and 5.0th day after radiation.Conclusion Transplantation of hMSCs can improve the survival rate and promote the repairation of intestinal injury induced by radiation in mice.MSCs are hopeful to be used on the treatment of radiation-induced intestinal injury.