目的 研究人外周血人五聚素3(PTX3)、可溶性CD40配体(sCD40L)及核因子-κB(NF-κB)与脑梗死的相关性,并进一步探讨此3种炎性因子之间的关联及可能的信号通路.方法 选取急性脑梗死患者84例作为病例组,及同时期体检中心健康体检者80名作为对照组.分别采用酶联免疫吸附测定法(ELISA)和流式细胞术(FCM)检测血浆sCD40L、PTX3的浓度,及人外周血单个核细胞(PBMC)中NF-κB的阳性表达率.结果病例组PTX3及sCD40L浓度、NF-κB阳性率均显著高于对照组(P<0.05).病例组NF-κB与PTX3表达水平之间存在正相关(r=0.252,P=0.021),sCD40L与PTX3血浆水平之间存在正相关(r=0.342,P=0.001).结论 NF-κB、sCD40L及PTX3均是与脑梗死密切相关的炎性因子,三者之间可能是相关炎症信号通路中的上下游炎症因子.%Objective To determine the correlation between human peripheral blood pentosan 3 (PTX3), solu-ble CD40 ligand (sCD40L), nuclear factor-κB (NF-κB) and cerebral infarction, and to further investigate the correla-tion between these three inflammatory factors and the possible signaling pathways. Methods Eighty-four patients with acute cerebral infarction were included as the case group and a contemporary cohort of 80 healthy subjects exam-ined in the physical examination center were included as the control group. The concentration of plasma sCD40L and PTX3, and the positive expression rate of NF-κB in human peripheral blood mononuclear cells (PBMC) were measured by enzyme-linked immunosorbent assay (ELISA) and flow cytometry (FCM), respectively. Results The concentration of PTX3 and sCD40L and the positive rate of NF-κB in the case group were significantly higher than those in the control group (P<0.05). There was a positive correlation between the expression levels of NF-κB and PTX3 in the case group (r=0.252, P=0.021), and there was a positive correlation between the sCD40L and PTX3 plasma levels ( r=0.342, P=0.001). Conclusion NF-κB, sCD40L and PTX3 are inflammatory factors, which are closely related to cerebral in-farction, and may be the upstream and downstream inflammatory factors in the relevant inflammatory signaling path-ways.
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