Real-time and label-free detection of biomolecular interactions by oblique-incidence reflectivity difference method

摘要

We successfully conduct the label-free and real-time detection of the interactions between epoxy groups and rabbit IgG and 5 CTT CAG GTC ATG AGC CTG AT 3 oligonucleotide,and between the hybridization of 5 CTT CAG GTC ATG AGC CTG AT 3 and its complementary 3 GAA GTC CAG TAC TCG GAC TA 5 oligonucleotide,by the oblique-incidence reflectivity difference (OI-RD) method.The dynamic curves of OI-RD signals,corresponding to the kinetic processes of biomolecular combination or hybridization,are acquired.In our case,the combination of epoxy groups with rabbit IgG and 5 CTT CAG GTC ATG AGC CTG AT 3 oligonucleotide need almost one and a half hours and about two hundred seconds,respectively;and the hybridization of the two oligonucleotides needs about five hundred seconds.The experimental results show that the OI-RD is a promising method for the real-time and label-free detection of biomolecular interactions.