Gender differences in development of renovascular hypertension

摘要

Aim The aim of this investigation was to evaluate gender differences in development of renovascular hy- pertension. Methods The experiments were performed with 10-week-old Sprague-Dawley rats which were prepared by the narrow side of the renal artery to development renovascular hypertension （2K1C）. After 32 weeks of regular feeding, rats were instrumented to determine blood pressure in anesthesia state. The blood was collecting for determi- nation of Anglland ET-1 by using ELISA kit. The thoracic aorta were isolated and snap frozen in liquid nitrogen. Ap- plications Western Blot method to detect tissue protein expression of angiotensin-converting enzyme （ACE1, ACE2）, and Angllreceptor（AT1 R, AT2 R）. Results SBP and DBP were higher in 2K1 C-females than in 2K1 C-males [ SBP： （ 172 ± 19. 2） vs （ 157 ± 16. 4） mmHg , P 〈0. 01. DBP： 138 ± 15.4 vs 128 ± 15.5, P 〈0. 05, n =20]. Plasma AngII were higher in 2K1C-males [ （ 11.2 ±2. 87） vs （9.60 ±3.51 ） ng · L^-1, P 〈0. 05] , and ET-1 were higher in 2K1C- females （38.77 ± 17.99 vs 33.76 ± 17.50 ng · L^-1, P 〈0. 05） . In thoracic aorta tissue, the protein levels of ACE1 and ACE2 were increased in 2K1C-females （ACE1 ： 1. 204 ＋0. 258 vsl. 182 ±0. 209, ACE2： 2. 431 ±0. 214 vs 1. 528±0.130, P 〈0.01）, AT1R were increased in 2K1C-males （0.955 ±0.201 vs 0.841 ±0.176, P 〈0.05）, ATzR were increased in 2K1C-females （2. 464±0. 142 vs 1. 431±0. 062, P 〈0. 01）. In kidney tissue, the protein levels of ACE1 were increased in 2K1C-females （2. 023 ＋0. 144 vs 1. 079±0. 103, P 〈0. 01）, ACE2 were increased in 2K1C- males （2. 496 ±0. 314 vs 1. 706±0. 214, P 〈0. 05）, AT2R and ATzR were increased in 2K1C-males （AT2R：0. 867 ±0. 092 vs O. 685±0. 176, P 〈0. 05, ATzR： 1. 413 ± 0. 233 vs 1. 146 ±0. 146, P 〈0. 05）. In heart tissue, the pro- tein levels of ACE1 were increased in 2K1 C-females （1. 122 ± 0. 177 vs O. 793± 0. 125, P 〈 0. 05） , ACE2 were no difference （0. 682 ±0. 108 vs O. 654 ±0. 103）, AT2R were increased in 2K1C-females （1. 478 ± 0. 235 vs 1. 223 ± 0. 193, P 〈0. 05）, ATzR were increased in 2K1C-males （ 1. 075 ± 0. 170 vs O. 924 ±0. 146, P 〈0. 05）. Conclu- sions The severity of hypertension was greater in 2K1 C-females than in 2K1 C-males. It is likely that the effect of fe- males on renovascular hypertension was depending on promoting the role of Ang II by increasing the release of ET-1,increasing the protein expression levels of ACE1 in thoracic aorta, kidney, and heart tissue, and increasing the pro- tein expression level of AT1 receptors in heart tissue.