EFFECT OF SUPERNATANT OF PERIPHERAL BLOOD MONONULEAR CELLS FROM NEPHROTIC PATIENTS ON THE SULFATED COMPOUND SYNTHESIS BY CULTURED GLOMERULAR EPITHELIAL CELL

摘要

To identify the role of circulating immune factor（s） in the development of proteinuria, the supernatant of peripheral blood mononuclear cells （PBMC） were obtained from 15 patients with steriod-responsive nephrotie syndrome, and its effect on the synthesis of sulfated compound by cultured rat glomerular epithelial cell （GEC） were studied by 35S, 3H-leucine dual-isotope labelling technique. The result showed that the supernatant of PBMC from 9 patients without steroid treat ment increased 35S uptake in GEC （P0.01）, while there was no significant difference in 35S uptake between the 6 patients treated with prednisone (1.5-2.0 mg/kg.d-1) and 6 normal controls.