Sialoadenitis progression in nonobese diabetic mice and its correlation with expression of apoptosis-associated proteins in salivary glands and serum IgG levels

摘要

＜正＞ Background Sjgren syndrome（SS）is an autoimmune disorder characterized by chronic lymphocytic infiltration anddecreased secretion in salivary glands.Apoptosis is one of the possible mechanisms involved in acinar epithelialdestruction in SS.The role of apoptosis in the initiation and effect phase of sialoadenitis is still controversial.The aim ofthis study was to observe the roles of apoptosis-associated proteins and serum IgG levels in sialoadenitis progression innonobese diabetic（NOD）mice.Methods 2-,5-,10-,15-,20-week female NOD and matched BALB/c control mice were selected.Saliva and tear flowrate were measured.Serum IgG level was tested by enzyme-linked immunosorbent assay（ELISA）.Number oflymphocyte foci（NLF）in submandibular glands（SMGs）was counted under routine hematoxylin/eosin-stained sections.Expression of Fas,Bcl-2 and procaspase3 proteins as well as apoptotic cells in the SMGs were detected byimmunohistochemical staining and by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling（TUNEL）assay respectively.Results Decreased stimulated total flow rate（STFR）and lymphocyte foci in SMGs were first observed in the 10-weekNOD group.STFR was negatively correlated with NLF（P＜0.05）.Serum IgG in NOD mice was significantly higher thanthat of the control group（P＜0.05）and showed a positive correlation with NLF（P＜0.05）.Fas expression in SMGs acinarcells in NOD mice increased with age and was significantly higher compared with that in the control group.Bcl-2expression and procaspase3 expression in SMG acinar cells in each NOD group were lower compared with those of theage-matched control mice.Conclusion Abnormal expression of Fas and Bcl-2 in the SMGs and higher level of serum IgG may contribute to theinitiation of sialoadenitis and cause the glandular destruction in NOD mice.Chin Med J 2007;120（16）:1426-1431