＜正＞ Background Promoter analysis is currently applied to detect the expression of the targeted gene in studies of signaltransduction and transcriptional regulation.As a reporter gene,luciferase plays an important role and has been usedwidely in the promoter assay.Methods Human embryonic lung fibroblast cells （2BS）,HeLa cells and MCF-7 cells were transfected with variousgenes embedded by lipofectamine.This study determined various factors that affect promoter activity determination,such as the selection of the reporter genes and internal references,the dose and the type of the vectors carrying thetranscription factors,the host cells and the instruments.Results The sensitivity of the luciferase assay was much higher than that of enhanced green fluorescence protein（EGFP）.Moreover,promoter activity is increased in a dose-related manner only in certain ranges outside of which theresults may be reversed and the promoter activity is related to the expression vector which is carrying the cDNA.Otherwise,the length of the promoter,internal references and the host cell can also influence the promoter activity.Conclusions To detect the promoter activity accurately,a few factors including dose,vector,length and host cell whichinfluence reporter gene assay aforementioned should be considered.
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