Analysis of characteristics of whole-genome of influenza A H1N1 virus in Qingdao between year 2009 and 2011

摘要

Objective To investigate characteristics of the whole-genome of influenza A H1N1 virus circulated in Qingdao from year 2009 to 2011.Methods RNA of 35influenza A H1N1 virus isolates circulated in Qingdao between year 2009 and 2011 was extracted and all segments were amplified by RT-PCR.The sequence was then detected and assembled by software Sequencher.25HA full-length sequences published on GenBank were selected as reference.While MEGA 5.0 software package was explored for