Complete nucleotide sequence of a human coxsackievirus B3 strain A103 /KM /09 isolated in Yunnan province,2009

摘要

Objective To analyze the genetic characterization of the complete genome from a human coxsackievirus B3strain A103/09 isolated in Yunnan province,2009.Methods By using RT-PCR,all the eight fragments containing about 1 000 nucleotides and covering full viral genome,were sequenced.By using Mega 5.05,Geneious,RDP 3 and SimPlot 3.5.1 software,sequences were aligned with other enterovirus reference sequences.Phylogenetic and recombination analysis were also carried out.Results The A103/KM/09