Establishment of animal models for chronic pulmonary embolism

摘要

Objective:To establish the animal model for the chronic pulmonary embolism and do some research on it.Methods:Pulmonary arteriography by DSA were performed in 6 goats and the stent was released in the infer-pulmonary arterary.Arterial blood gas analysis and right heart catheterization were done.After 3 days of embolism,pulmonary arteriography were performed again.After the animal was sacrificed,the right and left infer-pulmonary tissues were prepared and pathologically studied.Results:Stents were smoothly released in 6 animals.Compared with that before embolism,the arterial blood gas showed no difference 10 minutes later and 3 days later of the stent released.The pulmonary angiography showed that the right infra pulmonary artery was partly blocked and the blood could still pass by.3 d of the embolism later,the stent was placed well and the right pulmonary artery was completely blocked.Compared with that before embolism,the right atrial pressure,ventricular pressure and the pulmonary pressure in 10 min after embolism did not change much(P>0.05),while 3 d after embolism,the pressures were obviously changed(P<0.05).There was no atelectasis,pleural effusion in the right infra lobe in the experimental group.The wall of blood capillary among the alveolar wall were significantly dilated.Hyperemia and edema were also observed.Lots of monocyte infiltrated.Localized exudates appeared in alveolar.Conclusion:By releasing stent in the pulmonary,the chronic pulmonary embolism model could be established in the animal experiment,and it should have great potential value in the following study.