重组人γ-干扰素基因工程菌发酵工艺优化

摘要

研究不同类型培养基、诱导时间、菌体密度和不同补料对rhIFN-γ表达的影响,并运用高密度发酵工艺对rhIFN-γ的产量进行优化,目的蛋白表达量从4 000 mg/L提高到20 000 mg/L以上,发酵产量提高了5倍,大大提高了效率。同时为验证工艺的稳定性,将优化的发酵条件进行连续三批重复发酵实验。结果表明该优化条件具有重复性,表达稳定,维持在40%左右,从而为rhIFN-γ大规模生产奠定了良好的基础。%The expression level of rhIFN-γ was influenced by the different medium,induction time,cell density and feeding batch.The production of rhIFN-γ was optimized by high density fermentation.The expression level of rhIFN-γ was increased from 4 000 mg/L to over 20 000 mg/L,the fermentation yield was increased by 5 times.In order to confirm the technical stability,a three-time repeated fermentation was performed.The result indicated that the optimal conditions had the repeatability and the expression level remained 40% or so.This fermentation technology established a good foundation for the rhIFN-γ mass production.