香蕉枯萎病菌esyn1基因的克隆与序列分析

摘要

采用RT-PCR方法扩增了香蕉枯萎病菌恩镰孢菌素合成酶基因esyn1的cDNA片段,测序结果表明,esyn1基因核苷酸序列阅读框架全长546bp.核苷酸序列分析显示该基因片段编码181个氨基酸,推测分子量为19.9 ku,等电点为5.06.氨基酸序列比对结果表明,它与半裸镰刀菌、层出镰刀菌、拟枝孢镰刀菌esyn1基因的氨基酸序列的相似性分别为96％、94％、77％.%The esynl gene, encoding a multifunctional enzyme enniatin synthetase, was amplified by Reverse Transcription Pojymerase Chain Reaction. Sequence analysis showed the sequence of esynl gene was 346 bp and it encoded 181 amino acids, the molecular weight and theoretical isoelectric point was 19.9 ku and 5.06, respectively. The nucleotide identities of esynl compared with Fusarium proliferatum, Fusarium pallidoroseum and Fusarium poae, was 96%, 94% and 77%, respectively. These results would help to understand the function of esynl genes in infection process.