In order to study the regulation of Sly-miR 167a promoter, a 2 134 bp fragment upstream the transcription start was amplified from tomato genomic DNA by PCR, which was fused to GUS and transformed into tomato (cv Microtom). The temporal and spatial specificity during plant development were analyzed. To investigate the response to exogenous hormone, treatments were performed on the transgenic tomato. Promoter analysis revealed that some conserved regulatory sequences and several regulatory elements appeared in the promoter fragment. The results indicated that Sly-miR167a might express in all tissues, and express intensely in leaves, stems, flowers at anthesis and 2 days before anthesis, and fruits at immature green, mature green and breaker stages, and it was up-regulated by ethylene, NAA and GA. This result revealed that the expression of Sly-miR167a promoter showed temporal and spatial specificity, and it was regulated by exogenous hormone.%利用PCR技术从番茄基因组DNA中扩增了Sly-miR167a转录起始点上游2 134bp的片段,并将其与GUS基因融合构建植物表达载体,转化番茄,研究番茄Sly-miR167a启动子的时空表达特异性:并利用外源激素处理,研究其对激素的响应特性.启动子序列分析结果表明,Sly-miR167a启动子片段含有启动子调控的保守序列以及生长素、乙烯和赤霉素等响应元件.根据GUS染色结果推测,Sly-miR167a可能在番茄的各个组织中均有表达,并且在叶片、茎、开花前2d、开花当天、果实幼果期、绿熟期和破色期表达强烈.通过外源激素处理后,GUS基因的表达均有显著的上调.说明Sly-miR167a启动子的表达具有时间特异性,并且受外源激素的调控.
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