华南型黄瓜全雌基因的AFLP连锁标记开发及定位分析

摘要

以华南型全雌黄瓜B36为母本,弱雌黄瓜S6为父本,构建F2群体,利用AFLP分子标记技术对64对引物在F2群体上进行筛选,结果发现1条AFLP分子标记与全雌性状紧密连锁,且该连锁标记在F2代群体中呈3∶1质量性状分离.经生物学软件JoinMapV3.0分析显示,该标记与全雌基因的遗传距离为4.9 cM.此外,生物信息学分析表明,该标记位于黄瓜基因组第6号染色体末端.同时,在CsACS1G基因的远启动子区域设计1对引物,分别在B36和S6的F2代群体中进行PCR检测,结果显示,扩增片段与全雌性状无连锁关系.说明华南型全雌黄瓜B36的全雌性状受控于1个新的全雌基因.%In this experiment, F2 population was built by using the South China-type gynoecious cucumber, B36, as female parent and weak female cucumber, S6, as male parent. Sixty-four pairs of primers were screened in the F2 population and an amplified fragment length polymorphism (AFLP) molecular marker linked to the all female trait was found and the segregation ratio of female and weak female fitted 3:1 in F2 population, suggesting that gynoecy was a quality trait. The analysis results using the biology software, JoinMapV3.0, indicated that the genetic distance between the marker and gynoecious gene was 4.9 cM. In addition, Bioinformatics analysis showed that the marker was located in the terminal of the sixth number chromosome of cucumber genome. At the same time, we selected two pairs of primers in far promoter region of CsACSlG gene and test F2 plants from the progeny of B36 and S6 using a pair of primers by PCR. And the experiment results showed that there was no linkage between the amplified fragments and the gynoecious trait, which suggested that the gynoecious trait of South China-type gynoecious cucumber (B36) was controlled by a new gynoecious gene.