Preparation and physicochemical properties of scaffold materials of heterogeneous deproteinized bone

摘要

Objective:To prepare and observe the physicochemical properties of scaffold materials of heterogeneous deproteinized tissue-engineered bone.Methods:Deproteinized bone was made through a series of physicochemical treatments in pig ribs and analyzed with histological observation,scanning electron microscopy, infrared spectrum, X-ray diffraction and energy dispersive analysis, Kjeldahl determination and mechanics analysis.Results:Interstitial collagen fiber was positive and mucin was negative in deproteinized bone,but,both were positive in fresh bone. Deproteinized bone maintained natural pore network.Its pore size was 472.51 μm±7.02μm and the porosity was 78.15 % ± 6.45 %.The results of infrared spectrum showed that collagen was present in deproteinized bone.Both fresh and deproteinized bone had curve of hydroxyapatite.The Ca/P ratios were 1.71 ±0.95and 1.68 ± 0.76 ( P ＞ 0.05 ),and the protein contents were 26.6% ± 2.23% and 19.1% ± 2.14% (P ＜ 0.05)in fresh and deproteinized bone,respectively.There was no significant difference of destruction load under compression and maximal destruction load between fresh and deproteinized bone (P ＞ 0.05).The elastic modulus was higher in deproteinized bone than that in fresh bone (P ＜ 0.05).Conclusions:Physicochemical properties and mechanic strength of deproteinized tissue-engineered bone meet the demands of ideal scaffold materials. But,its immunogenicity should be observed through further experiments for its clinical applications.