BACKGROUND:Scholars have made certain progress in the basic and clinical studies regarding antibacterial bone graft materials. OBJECTIVE: To investigate the mechanical property of antimicrobial silver-loaded coral hydroxyapatite bone usingin vitro mechanical experiments and to explore its ability to repair large segmental contaminative bone defects. METHODS:Compression test and three-point bending test were used to evaluate the mechanical properties of silver-load coraline hydroxyapatite, coraline hydroxyapatite and coral. Thirty-six New Zealand white rabbits were selected and randomly divided into four groups to establish right-side large segment of contaminative radial bone defect models. Rabbits in three groups were implanted silver-load coraline hydroxyapatite, coraline hydroxyapatite andin situ autologous bone, and rabbits in the other group were not implanted any material (as control). At 2, 6 and 10 weeks post-operation, the rabbits were sacrificed to take specimens. The repair of bone defects in each group was observed and compared by gross observation, radiographic examination and histological examination. The antimicrobial condition in each group was evaluated by bacteriological examination. RESULTS AND CONCLUSION:There was no significant difference between the mechanical properties of silver-load coraline hydroxyapatite, coraline hydroxyapatite and coral. At the 10th week post-operation, X-ray and histological observation showed mature bone tissues in the silver-load coraline hydroxyapatite bone group. A large number of lacunae and mature bone cels were visible in bone tissues. Haversian system was visible. Most of the materials were degraded, and there was only a smal amount of residual material. Partial recanalization was visible in bone marrow cavity. The repair effect of silver-load coraline hydroxyapatite bone group was similar with the autologous bone group, and better than the coraline hydroxyapatite group and the control group. Bacteriological examination showed that the silver-load coraline hydroxyapatite had good antibacterial ability. These results demonstrate that the silver-load coraline hydroxyapatite has good mechanical properties and antibacterial properties, which can be used to repair large segmental contaminative bone defects.%背景：国内外学者在抗菌骨移植材料的基础实验和临床实践方面进行了大量的研究，取得了一定的进展。目的：通过体外力学实验检测抗菌性载银珊瑚羟基磷灰石人工骨的机械性能，并通过动物实验检测其修复大段污染性骨缺损的能力。方法：利用压缩试验及三点弯曲试验评价载银珊瑚羟基磷灰石、珊瑚羟基磷灰石与珊瑚的力学性能。将36只新西兰大白兔随机分为4组，均制作右侧大段污染性桡骨缺损模型，其中3组分别植入载银珊瑚羟基磷灰石、珊瑚羟基磷灰石与原位自体骨，另1组不植入任何材料(对照)。术后2，6，10周处死动物并取材，通过大体观察、影像学检查、组织学检查观察比较各组骨缺损修复情况，通过细菌学检查评估各组抗菌情况。结果与结论：载银珊瑚羟基磷灰石、珊瑚羟基磷灰石与珊瑚的力学性能无差异。术后10周时，X射线及组织学观察结果显示，载银珊瑚羟基磷灰石骨缺损内为成熟的骨组织，骨组织中有大量的骨陷窝和成熟的骨细胞，可见哈弗氏系统，材料大部分降解，只有少量残留，骨髓腔有部分再通，骨缺损修复效果与自体骨组相似，优于珊瑚羟基磷灰石组与对照组。细菌学检查显示载银珊瑚羟基磷灰石具有良好的抗菌能力。表明载银珊瑚羟基磷灰石具有较好的力学性能及抗菌性能，可用于修复大段污染性骨缺损。
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