脂肪干细胞在颗粒脂肪移植中的作用

摘要

背景：血管基质层细胞中含有大量的脂肪干细胞，有研究显示脂肪干细胞在移植过程中可能发挥着很重要的作用。　　目的：观察脂肪干细胞在颗粒脂肪移植中的作用。　　方法：选取10只雄性SPF级BALB/C小鼠，取出正常脂肪组织，提取动物腹部脂肪中的脂肪干细胞和颗粒脂肪，选取24只裸鼠作为移植受体，所有动物分为3个实验组，分别为对照组、颗粒脂肪移植组、混合移植组(脂肪干细胞和颗粒脂肪)。2个移植组分别将颗粒脂肪细胞悬液及脂肪干细胞和颗粒脂肪混匀后细胞悬液注射到小鼠腹背左右肩胛处；对照组注射等量的细胞基础培养液。4周后，分离血浆及取出移植物进行指标检测。　　结果与结论：①混合移植组能显著性的增加移植物的质量，降低移植脂肪的吸收率，相对于颗粒脂肪移植组差异有显著性意义(P<0.01)；②移植后血浆中血管内皮细胞生长因子相对于对照组明显提升，其中混合移植组高于颗粒脂肪移植组(P<0.01)；③颗粒脂肪移植组碱性成纤维细胞生长因子的含量明显低于相混合移植组；④混合移植组微血管密度显著高于颗粒脂肪移植组(P<0.01)；⑤动物移植物中的细胞形态较单纯颗粒脂肪细胞完好；⑥混合移植组的脂肪油滴的数目明显多于颗粒脂肪移植组；⑥结果提示，脂肪干细胞能够显著性提高碱性成纤维细胞生长因子的表达，改善移植物的微循环，显著改善颗粒脂肪细胞的形态和功能。%BACKGROUND:There are a lot of adipose-derived stem cel s in the vascular stroma. These cel s are shown to play a very important role in the fat granule transplantation. OBJECTIVE:To explore the role of adipose-derived stem cel s in the fat granule transplantation. METHODS:Normal adipose tissues were obtained from 10 male BALB/C mice, SPF grade. Adipose-derived stem cel s and fat granules were extracted from the abdominal fat tissues. Another 24 nude mice acted as recipients and were assigned into control, fat granule transplantation or mixed transplantation (adipose-derived stem cel s+fat granules) groups. In the latter two groups, fat granule suspension and suspension of fat granules and adipose-derived stem cel s were injected into the shoulder of rats, respectively. In the control group, the same volume of cel medium was injected. Four weeks later, separated plasma and grafts were taken out for indicator measurement. RESULTS AND CONCLUSION:Compared with the fat granule transplantation group, the mixed transplantation could remarkably increase the weight of grafts, while reduce the absorption of grafted fat tissues (P<0.01). After transplantation, the highest level of vascular endothelial growth factor in the plasma was obtained in the mixed transplantation group fol owed by fat granule transplantation group and control group (P<0.01). Level of basic fibroblast growth factor and microvessel density were significantly higher in the mixed transplantation group than the fat granule transplantation group (P<0.01). Better cel morphology and higher number of fat droplets were found in the mixed transplantation group compared with the fat granule transplantation group. Al these results indicate that adipose-derived stem cel transplantation can remarkably promote the expression of basic fibroblast growth factor, improve graft microcirculation, and improve morphology and function of fat granules.