心血管补片在先天性心脏疾病中的研究与应用前景

摘要

背景:组织工程心血管补片在外科手术治疗先天性心脏病的解决方案中起着决定性的作用.组织工程心血管补片构建策略灵活丰富,基础研究和临床应用范围广泛.目的:对心血管补片在先天性心脏病外科手术治疗中的应用进展以及组织工程心血管补片的构建方法进行综述.方法:采用电子检索方式检索PubMed数据库、Medline数据库、中国期刊全文数据库、临床试验注册资料库2001年1月至2016年12月收录的有关组织工程心血管补片分类、设计研究以及基础和临床研究的文献.以"cardiac tissue engineering,pericardium patch,stem cel,congential heart disease,congenital heart conditions,CHD"为英文检索词,以"心脏补片,血管补片,心血管补片和先天性心脏病"为中文检索词.最终纳入32篇文献进行分析.结果与结论:随着人类干细胞生物学、材料学和检测技术的进步,组织工程心血管材料有望实现标准化和产业化管理.但随之而来的问题也有很多,例如:三维血管设计策略的具体实施方法,多种种子细胞联合搭载的比例控制,临床前质控和产业化管理标准等,紧密的国际间合作能够推动整体产业健康良好地发展.%BACKGROUND: Tissue-engineered cardiovascular patch plays a decisive role in the surgical treatment of congenital heart diseases. Its construction strategies are rich and flexible. This kind of patch has a wide range of application in basic and clinical research.OBJECTIVE: To review some progresses in the surgical application of cardiovascular patch in congenital heart disease and the methods to construct the tissue-engineered cardiovascular patch.METHODS:PubMed, Medline, CNKI and ClinicalTrials.gov were searched by computer for articles related to classification, design, basic and clinical research of tissue-engineered cardiovascular patch were published between January 2001 and December 2016. The keywords were "cardiac tissue engineering, pericardium patch,stem cell, congenital heart disease, congenital heart conditions, CHD" in English and "cardiac patch, vascular patch, cardiovascular patch, congenital heart disease" in Chinese. Finally, 32 articles were included in result analysis.RESULTS AND CONCLUSION: With the development of human stem cell biology, materials science and testing technology, the tissue-engineered cardiovascular material is expected to achieve standardization and industrialization management. However, subsequent problems need to be solved, for example, the specific implementation of a three-dimensional vascular design strategy, the loading proportion of a variety of seed cells,pre-clinical quality control and industrial management standards, as well as the close international cooperation that can promote the overall healthy development of the industry.