OsBTF3过量表达和RNAi转基因水稻抗盐和抗低温胁迫鉴定

摘要

为评估水稻OsBTF3基因在水稻对非生物逆境胁迫应答中的功能,利用RT-Q-PCR技术,对经高盐(200mmol/L NaCl溶液)和低温(4℃)胁迫处理后水稻的OsBTF3基因表达动态进行了测定,并对前期获得的OsBTF3过量表达和RNAi转基因水稻株系进行了抗高盐和抗低温胁迫的鉴定.结果表明,高盐胁迫处理显著抑制了OsBTF3基因的表达,低温胁迫对其表达影响有一定的变化；转基因T2代过量表达株系对高盐和低温胁迫抗性显著增强,而RNAi转基因株系抗性减弱,说明OsBTF3可能在水稻对高盐和低温胁迫反应中起着重要的调控作用.%To demonstrate the biological function of UsBTF3 gene in rice resistance or tolerance to salt and cold stress, the gene transcription of OsBTF3 responsive to salt and cold treatments was quantified via real-time PCR assay, and resistance to the stress of transgenic rice seedlings with over-expressed and RNAi-silenced OsBTF3 was analyzed. The results showed that gene expression of OsBTF3 was significantly decreased in rice plants under salt stress, while lightly reduced under cold stress. The resistance to salt and cold stress was enhanced highly in the seedlings of transgenic lines with over-expressed OsBTF3, while decreased in the RNAi-silenced 0sBTF3 lines. The results revealed that OsBTF3 might function as a key regulator for abiotic stress resistance in rice.