Separation and purification of human chorionic gonadotropion(HCG) in the urine sample of early pregnant women by D3520 resin adsorption chromatography is reported.The crude product obtained by DEAE-Cellulose 23 and DEAE-Sephadex A50 column chromatography showed a high activity of HCG. Further purification of the sample by gel filtration chromatography on a Sephadex G75 column gives a final preparation of 6000-6500 IU/mg.The preparation meets the requirements of the pyrogn test in Chinese Law of Pharmacopeia.
展开▼
机译:报道了采用D3520树脂吸附色谱法分离和纯化早期孕妇尿液中人绒毛膜促性腺激素(HCG),DEAE-Cellulose 23和DEAE-Sephadex A50柱层析法得到的粗产物显示高HCG活性。在Sephadex G75色谱柱上通过凝胶过滤色谱法进一步纯化样品,最终制备的样品为6000-6500 IU / mg。该制剂符合《中国药典》中焦酚测试的要求。
展开▼