＜正＞ Macroporous methyl acrylate - divinyl benzene copolymers were synthesized and modified by polyethylene polyamine. The aminoacylase from Aspergillus o-ryzae was adsorded on these modified copolymers. The role of certain factors such as degree of cross-linking, amount of poregenic solvent, modifying agent and pore structures, in the activity of immobilized aminoacylase was studied. Effects of adsorption time and amount of enzyme used on the activity of aminoacylase immobilized on MMD-12-13 were also investigated. The column of aminoacylase immobilized on MMD-12-13 was prepared and N-acyl-DL-methionine was resolved continuously for a month, the remaining activity was still over 90%.
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