KLF6在帕金森病体外模型中对多巴胺能神经元存活的影响

摘要

目的：在帕金森病体外模型的多巴胺能神经元中，探讨KLF6的变化及意义。方法用100uM 的MPP＋处理多巴胺能细胞系SN4741细胞，制造帕金森病体外模型。用实时定量PCR和western blot检测KLF6的mRNA以及蛋白水平的变化。通过慢病毒感染在 SN4741细胞中过表达 KLF6，然后用 western blot 检测病毒感染后 KLF6的表达量。在SN4741细胞中过表达KLF6后，用100uM 的MPP＋处理对照组和过表达KLF6组的细胞，通过流式细胞仪和MTT检测细胞凋亡情况。结果（1）和对照组相比，MPP＋处理后的SN4741细胞中KLF6 mRNA和蛋白水平的表达量显著下降（P＜0.05）。（2）过表达KLF6的病毒感染SN4741细胞后，KLF6的蛋白水平显著增加（P＜0.05）。（3）用100 uM 的MPP＋处理对照组和过表达KLF6组，和对照组相比，KLF6过表达组的细胞凋亡比例显著下降（P＜0.05）。结论 KLF6在帕金森病体外模型中起到保护多巴胺能神经元的作用，促进神经元存活，为治疗帕金森病提供了新的靶点和思路。%Objective To explore the change of KLF6 and its significance in the dopaminergic neurons of the Parkinson's disease in vitro model. Methods In vitro model of Parkinson's disease were made by treating SN4741 cells with 100 μM MPP+ .Then qRT-PCR and western blot were used to examine the change of KLF6 mRNA and its protein level. After that ,lenti-virus were used to make KLF6 over-express in SN4741 cells. After lentivirus infection ,KLF6 protein level was examined with western blot. Finally ,Flow cytometry and MTT were used to to examine cell apoptosis. Results Compared with the control group ,KLF6 mRNA and protein level were significantly decreased in the MPP + treated group (P<0.05). Lentivirus infec-tion led to a significant increase of KLF6 protein level in SN4741 cell.Up-regulated KLF6 obviously protected SN4741 cells from MPP+. Conclusion KLF6 can protect dopaminergic neurons from MPP+ ,which will provide a new target for the treat-ment of Parkinson's disease.