高成脂脂肪干细胞系的分子克隆筛选

摘要

目的 探讨并筛选具备高成脂能力的脂肪干细胞系表面标志、可应用于脂肪组织工程的种子细胞,以提高组织工程化脂肪的构建效率.方法 胶原酶消化人脂肪组织,获得脂肪干细胞,培养扩增后成脂诱导,收集诱导成熟的脂肪细胞,天花板贴壁培养得到去分化脂肪细胞.比较去分化脂肪细胞与人脂肪干细胞的增殖能力,成脂分化能力及表面抗原表达的变化.结果 去分化脂肪细胞与脂肪干细胞的形态和增殖能力相似;去分化脂肪细胞的成脂分化能力高于脂肪干细胞;两种细胞表面抗原的表达大致相同,但去分化脂肪细胞的CD54的阳性表达高于脂肪干细胞.结论 CD54的表达可能与去分化脂肪细胞的高成脂分化能力密切相关,可能是高成脂系干细胞的特异性表面抗原标志.%Objective To investigate the sttrface markers of adipose-derived stromal cells(ASCs) with high potential of adipogenic differentiation for increasing efficiency of adipose tissue engineering with selected seeds cells.Methods ASCs were harvested from human adipose tissue by collngenase digestion.After proliferation and adipogenic induction of ASCs,the mature induced adipocytes floating in the induction medium were collected.Ceiling culture was used to cluture adipocytes and then dedifferentiated adipocytes was obtaind et the ceiling.The reproductive activity,adipogenic differentiation potency and expression of surface markers were compared between the dedifferentiated adipocytes and ASCs.Results The reproductive activity between the dedifferentiation adipocytes and ASCs were similar.The potential of adipogenic differentiation of the dedifferentiated adipocytes was stronger than that of the ASCs.The expression of cell surface markers of both cells were almost the same.But the CD54 positive expression in dedifferentiated adipocytes was higher than that in ASCs.Conclusion The CD54 expression maybe closely associated with high potential of adipogenicdifferentiation of dedifl'erendated adipecytes.CD54 maybe the specific identification of cell surface marker of ASCs with high adipogenic differentiation.