Objective To explore the migration of intravenously injected adipose tissue-derived stem cells(ADSCs) in SD rats with soft tissue wound. Methods 1.8cm×1.8cm full thickness skin defect and 0.5cm in depth soft tissue defects were made on the back of 6 SD rats. ADSCs were isolated and cultured in vitro for 3 passages. 2.4×10~6 ADSCs were labeled with DiI and transplanted into the SD rats through taft vein. Nounal skin and wound tissue Samples were collected for fluorescent distribution observation 24、48 days after injection, respectively. Results Compared to normal skin, more florescent positive cells were detected in the margin and deep layer of the wound 24 days after operation. But it is accumulated within dermis and adenoid 48 days after operation. Conclusion Wound can probably induce the migration and accumulation of intravenously transplanted ADSCs.%目的 研究静脉注射后的脂肪来源干细胞向大鼠创伤部位的迁移和聚集情况.方法 取SD大鼠6只,制备1.8 cm×1.8 cm范围的皮肤全层及0.5 cm深度的皮下软组织缺损的创伤模型.取2.4×10~6个SD大鼠第3代脂肪来源干细胞经DiI标记后通过尾静脉注射到创伤大鼠体内,分别于移植后24、48 d分批处死大鼠,获得各时间点的创面愈合组织及健康组织标本,冰冻切片后荧光显微镜下观察荧光分布,同时组织石蜡包埋后行切片、HE染色.结果 与健康皮肤组织比较,24 d时在创面愈合组织周边和皮下组织深层部位可见较强的荧光分布,48 d时结合组织病理学观察可以发现在真皮层、腺样结构组织内荧光较强.结论 创伤可定向诱导大鼠脂肪来源干细胞在体内的迁移和集聚.
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