TSP-1与NF-κB通路蛋白在婴幼儿毛细血管瘤中的表达

摘要

目的 通过检测不同分期血管瘤中凝血酶敏感蛋白-1(thrombin sensitive protein-1,TSP-1)和核因子κB (nuclear factor kappa B,NF-κB)通路相关蛋白表达及其与微血管密度microvessel density(MVD)关系,探索其在血管瘤增生和消退机制中的意义.方法 随机选取血管瘤患儿40例,据Mulliken标准分为增生期组21例,消退期组19例.采用免疫组化法检测对比增生期及消退期血管瘤内皮细胞中TSP-1、CD36、p59fyn、Caspase3、p38MAPK、NF-κBp65、p-IκBα、p-IKKβ、VEGF表达和血管内皮标志抗原CD34表达;对比分析TSP-1、NF-κB通路中各蛋白表达与微血管密度相关性.结果 TSP-1和CD36、p59fyn、Caspase3、p38MAPK各指标在增生期表达均低于消退期(F=32.582、47.575、11.645、18.824、13.140;P =0.000、0.000、0.002、0.000、0.001).NF-κBp65和p-IκBα、p-IKKβ、VEGF各指标在增生期表达均高于消退期(F=7.807、6.323、5.988、4.216;P=0.009、0.018、0.021、0.049).增生期微血管密度高于消退期(F =7.256,P=0.012).瘤体中TSP-1、CD36、p59fyn表达与MVD表达均呈负相关(rs=-0.420、-0.519、-0.388;P=0.021、0.003、0.034).NF-κBp65、p-IκBα、VEGF表达与MVD表达均呈正相关(rs=0.666、0.611、0.515;P=0.000、0.000、0.004).TSP-1与NF-κBp65呈负相关(rs=-0.413,P=0.023).结论 TSP-1和NF-κB信号通路在婴幼儿毛细血管瘤的病程演变中可能相互调控,通过影响瘤体血管生成而对其增生和消退发挥重要作用.%Objective To explore the significance of the relative proteins in thrombin sensitive protein(TSP-1) and nuclear factor kappa B (NF-κB) signal pathways by detecting their expressions in different phases of hemangioma and the relations between them and microvessel density(MVD).Methods Forty cases with hemangioma were randomly selected and divided into proliferative group(n =21) and involutional group(n =19) according to Mulliken standard.The expressions of TSP-1,CD36,p59fyn,Caspase-3,p38MAPK,NF-κBp65,p-IκBα,p-IKKβ,VEGF and CD34 in endothelial cells were detected by immunohistochemistry.The relevance between the proteins in TSP-1 and NF-κB signal pathways and MVD were compared and analyzed respectively.Results The expressions of TSP-1,CD36,p59fyn,Caspase-3,p38MAPK in proliferative hemangioma were all lower than those in involutional hemangioma (F=32.582,47.575,11.645,18.824,13.140;P =0.000,0.000,0.002,0.000,0.001).Conversely,the expressions of NF-κBp65,p-IκBα,p-IKKβ,VEGF in proliferative hemangioma were higher than those in involutional hemangioma(F =7.807,6.323,5.988,4.216;P =0.009,0.018,0.021,0.049).Meanwhile,MVD were different between the two groups(F =7.256,P =0.012).The expressions of TSP-1,CD36,p59fyn and MVD showed a negative relationship(rs =-0.420,-0.519,-0.388;P =0.021,0.003,0.034).Conversely,the expressions of NF-κBp65,pvIκBα,VEGF and MVD showed a positive relationship(rs =0.666,0.611,0.515;P =0.000,0.000,0.004).The expression of TSP-1 and NF-κBp65 showed a negative relationship (rs =-0.413,P =0.023).Conclusions TSP-1 signal pathway and NF-κBp65 signal pathway may be interactive in evolution of infantile hemangioma,which has a potential role by effecting the progress of angiogenesis.