GC-MS 蒙古苍耳挥发油成分分析

摘要

目的：对蒙古苍耳挥发油成分进行分析研究。方法采用超声波提取法提取蒙古苍耳中的挥发性成分，GC 毛细管柱色谱法进行分离，质谱检测器进行分析，峰面积归一化法确定其相对含量，气相色谱-质谱联用技术辅助人工检索鉴定其化学成分。结果从蒙古苍耳的挥发油中分离鉴定出23种化学成分，主要成分有异硫氰酸甲酯（2.92%），双（三甲基硅基）雌二醇（1.85%），维生素 E（1.33%），五味子醇甲（1.78%），秋酰胺（3.09%），谷甾醇（1.63%），4,4-二甲基胆甾（15.54%），秋水仙碱（1.86%），3,7,11,15-四甲基-十六醇（13.34%），α-亚麻酸（16.02%），γ-亚麻酸（30.12%），粪甾烯（2.56%），碱性艳绿（4.49%），豆甾醇（1.09%），上述14种成分含量占总含量的99.7%。结论蒙古苍耳挥发性成分中亚麻酸总含量最高，占挥发性成分总量的46.14%。%Objective To analyze the volatile oil constituent of Xanthium mongolicum Kitag. Methods The volatile components were extracted by ultrasonic extraction, analyzed by capillary GC. The relative content of chemical components was calculated by peak area normalization method, the chemical components were identified by gas chromatography mass spectrometry(GC-MS). Results Twenty-three components were identified, the main identified chemical components account for 99.97% of the total volatile components, including isothiocyanato methane(2.92%), bis(trimethylsilyl)estradiol(1.85%), tocopherol(1.33%), schizandrin(1.78%), hiceinamide(3.09%), sitosterol(1.63%), 4,4-dimethylcholestan(15.54%), colchicin(1.86%), 1-hexadecyn-3-ol, 3,7,11,15-tetramethyl-(13.34%),α-linolenic acid(16.02%), γ-linolenic acid(30.12%), coprostene(2.56%), basic green 1(4.49%), stigmasterol(1.09%). Conclusion The main chemical component of Xanthium mongolicum Kitag volatile components was linolenic acid, accounting for 46.14% of the total volatile components.