基于大鼠急性心肌缺血模型冠心丹参方有效组分配伍作用评价

摘要

目的：观察冠心丹参方对垂体后叶素致急性心肌缺血模型大鼠的保护作用，并对其有效组分配伍进行评价。方法采用舌下静脉注射垂体后叶素致急性心肌缺血模型的方法，观察不同配伍药物对注射垂体后叶素后不同时间点大鼠心电图 ST 段、T波的位移值，急性心肌缺血梗死范围及心肌组织的病理学检查。结果冠心丹参方中药物不同组分配伍均能明显对抗垂体后叶素引起急性心肌缺血大鼠心电图 ST 段、T 波的变化，能降低急性心肌缺血梗死范围、保护心肌细胞。冠心丹参方组对心肌缺血的保护作用最佳。结论冠心丹参方中药物不同组分配伍具有显著对抗垂体后叶素致大鼠急性心肌缺血的作用，其效应强度为：冠心丹参方>丹参+三七>丹参+降香>丹参>三七+降香。有效组分配伍的药效结果符合中药方剂配伍原则。%Objective To observe the protective effects of Guanxin danshen formula on pituitrin-induced acute myocardial ischemia in rats and to evaluate Guanxin danshen formula by combining with effective components. Methods The rat model of acute myocardial ischemia was established by injecting pituitrin into sublingual vein. The effects of medicine on electrocardiogram (ECG) ST segment and T wave in rats in different time after pituitrin injection were observed. Myocardial infarction size (MIS) of model rats was calculated and pathological examination of myocardial tissue was performed. These indexes were used to compare the difference of protective effects of Guanxin danshen formula by combining with effective components. Results Changes of ECG ST segment and T wave of pituitrin-induced acute myocardial ischemia rats were obviously inhibited by combining with effective components of Guanxin danshen formula. MIS was reduced and myocardial cells were protected. The Guanxin danshen formula group was the best to protect myocardial ischemia. Conclusion Guanxin danshen formula by combining with effective components can significantly inhibit acute myocardial ischemia in rats caused by pituitrin. The sequence of potency is as follows: Guanxin danshen formula, Danshen (Radix Salviae Miltiorrhizae) and Sanqi (Radix Notoginseng), Danshen and Jiangxiang (Dalbergia odorifera), Danshen, Sanqi and Jiangxiang. The results of effective components combinations accord with the compatibility of formula.