凤尾草醇提取物对α-萘异硫氰酸酯诱导的小鼠胆汁淤积型黄疸的保护作用

摘要

目的 考察凤尾草醇提取物对 α-萘异硫氰酸酯(α-naphthalene isothiocyanate,ANIT)诱导的小鼠胆汁淤积性黄疸的退黄保肝作用.方法 ICR小鼠连续灌胃给予凤尾草醇提取物125、250和500 mg·kg-15天,每天给药1次,在第3天给药后2 h单次灌胃给予ANIT 80 mg·kg-1,继续给药,末次给药前动物禁食不禁水12 h,末次给药后2 h处理动物.全自动生化分析仪检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、白蛋白(ALB)、碱性磷酸酶(ALP)、总胆红素(TBil)、直接胆红素(DBil)和胆汁酸(TBA)水平,取肝组织做病理检查.结果 与空白对照组相比,ANIT模型组动物血清转氨酶ALT、AST水平显著升高,ALB含量显著下降,TBil、DBil及TBA和ALP水平显著升高,肝脏组织病理损伤严重.凤尾草醇提取物125、250、500 mg·kg-1能显著降低血清转氨酶水平,升高白蛋白水平,同时显著降低血清胆红素、TBA及ALP含量.HE染色结果显示,凤尾草醇提取物抑制肝组织细胞的变性、坏死及炎症细胞浸润.结论 凤尾草醇提取物对ANIT引起的胆汁淤积性肝损伤及黄疸具有显著保护作用.%Objective To investigate the protective effects of alcohol extracts from Pteris multifida Poir (EPMP) on cholestatic jaundice induced by α-naphthalene isothiocyanate (ANIT) in mice. Methods ICR mice were given EPMP 125, 250 and 500 mg·kg-1 for 5 days orally, once per day. 2 h after administration of medication on the 3rd day, the mice were given ANIT 80 mg·kg-1 orally once. All of the animals were fasted 12 h after the final administration of EPMP, and mice were treated to collect livers and blood. The levels of serum ALT, AST, ALB, ALP, TBIL, DBIL and TBA were measured by automatic biochemical analyzer, and liver tissues were taken for pathological examination. Results ANIT 80 mg·kg-1 significantly increased the activities of plasma ALT, AST, ALP, TBil, DBil, TBA, decreased the levels of ALB and injured liver compared with control group. Upon EPMP 125, 250 and 500 mg·kg -1, the transaminase activity was markedly decreased and the level of ALB was elevated. At the same time, serum bilirubin, TBA and ALP levels were significantly reduced. The HE staining results demonstrated that EPMP remarkably attenuated liver damage including degeneration, necrosis and infiltration of inflammatory cells. Conclusion The extracts from Pteris multifida Poir have significant protective effects on ANIT-induced liver injury and cholestatic jaundice.