长链非编码 RNA MALAT1调控 Rac1 b表达与结直肠癌侵袭和转移的关系

摘要

目的：探讨MALAT1调控Rac1b的表达与结直肠癌侵袭和转移的关系。方法：结直肠癌患者组织样本手术切除后30 min内于－80℃保存；实时荧光定量PCR检测肿瘤组织和配对正常组织中MALAT1和Rac1b mRNA的表达；RNA干扰沉默结直肠癌细胞MALAT1表达，通过Western blot 检测Rac1b和上皮间质化标志物的表达，EdU实验检测其对细胞增殖的影响；Transwell小室实验检测MALAT1低表达后对细胞迁移和侵袭的影响。结果：MALAT1在结直肠癌中低表达；干扰MALAT1表达后，Rac1b的表达升高，细胞增殖加快，且上皮间质化标志物E－cadherin和β－catenin表达升高。干扰MALAT1的表达可明显促进结直肠癌侵袭和细胞的迁移和侵袭。结论：MALAT1低表达与结直肠癌侵袭和转移密切相关，并且这一相关性是通过调控Rac1b的表达实现的。%AIM:ToinvestigatetheroleofMALAT1incolorectalcancermetastasis.METHODS:ThemRNA expression levels of MALAT1 and Rac1b in the tumor and adjacent normal tissues were examined by real-time PCR. MALAT1 was knocked down by siRNA in colorectal cancer cell lines .The expression of Rac1b and the epithelial-mesen-chymal transition markers was examined by Western blot .Cell proliferation was determined by EdU analysis .The effects of MALAT1 on the cell migration and invasion were examined by Transwell assay .RESULTS: The expression of MALAT1 was down-regulated in colorectal cancer .Down-regulation of MALAT1 induced Rac1b overexpression, which in turn in-creased the expression levels of E-cadherin and β-catenin.Furthermore, down-regulation of MALAT1 promoted the cell proliferation, invasion and migration.CONCLUSION:MALAT1 is associated with metastasis of colorectal cancer through regulating the expression of Rac1b and the downstream factors.