目的 研究糖尿病大鼠骨质疏松发病过程中骨桥蛋白的表达.方法 24只雄性Wistar大鼠随机分成2组,糖尿病大鼠组12只,空白对照组12只,饲养4月后处死动物,检查骨桥蛋白mRNA水平表达.结果 糖尿病组骨桥蛋白总RNA提取及RT-PCR、琼脂糖凝胶电泳显示,糖尿病组大鼠在骨质疏松发病过程中,骨桥蛋白在骨组织中高表达,与空白对照组相比,差异具有显著性.结论 骨桥蛋白在糖尿病大鼠骨组织有显著性改变,推测骨桥蛋白参与调节骨质疏松发病过程.%Objective To investigate the expression of osteopontin (OPN) during the genesis of osteoporosis in diabetes rats. Methods Twenty^bur healthy male Wistar rats were randomly divided into two groups; the diabetic rat group and the blank control group. Each group had 12 rats. All the rats were executed after being raised for 4 months and the expression of OPN mRNA was examined. Results The total RNA extraction, RT-PCR, and agarose gel electrophoresis showed that OPN in diabetic rat group was overexpressed during the genesis of osteoporosis . The difference was significant compared with that in blank control group. Conclusion The level of OPN is significantly changed in the bone tissue of diabetic rats , and we speculate that OPN may participate in the regulation of the genesis of osteoporosis
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