扫描电镜法评价护理液对软性接触镜上蛋白的清除效果

摘要

Objective:To evaluate the ability of scanning electron microscopy (SEM) and image computer statistical analysis (ICSA) to quantitatively assess the effectiveness of protein removal from soft contact lenses by soft contact lens care solutions.Methods:Soft contact lenses were soaked in artificial tears for 65 hours to simulate protein adsorption.Two contact lens care solutions and phosphate buffered saline (PBS) were then used separately to rub,rinse,and soak the lenses for 6 hours.After fixation with glutaraldehyde,the lenses were dried.The morphology and quantity of protein on the surface of the lenses were inspected by SEM and ICSA.The cleansing rate was expressed as a mass fiaction,i.e.,a percentage of before and after treatment with the care solution.Single factor analysis of variance was used to analyze the protein clearance rate of lens treated with different treatments by Tukey multiple comparison method.Results:The contact lens care solution had a significant cleaning effect on the surface protein of the lenses.For PBS and the two kinds of contact lens care solutions from different manufacturers,the protein clearance rates of the lenses were 89.58％,98.18％ and 98.38％ at 100× magnification and 89.76％,98.42％ and 98.27％ at 1 500× magnification.Conclusion:SEM combined with ICSA can effectively evaluate the protein cleaning efficacy of contact lens care solutions.Contact lens care solutions have a significant cleaning effect on the surface protein of the lenses.%目的:通过扫描电镜观察并结合图像计算机统计分析法评价护理液对软性接触镜上吸附蛋白的清除效果.方法:实验研究.将软性接触镜镜片浸泡在人工泪液中65 h,模拟蛋白吸附,再分别用隐形眼镜护理液和PBS对镜片进行揉搓、冲洗和浸泡处理(6 h)操作;然后镜片经戊二醛水溶液固定后干燥,通过扫描电镜观察镜片表面的蛋白形态及数量,最后通过图像计算机统计分析法对镜片表面的蛋白数量进行定量分析,吸附蛋白的软性接触镜经护理液处理前后蛋白数量减少值占镜片处理前表面蛋白的百分比,即为清除率.采用Tukey多重比较法对不同护理液处理的镜片表面蛋白清除数据进行单因素方差分析.结果:隐形眼镜护理液对镜片表面吸附的蛋白有显著清除效果,100倍放大倍数下,PBS对镜片表面蛋白的清除率为89.58％,2个不同厂家的隐形眼镜护理液对镜片表面蛋白的清除率分别为98.18％和98.38％;1 500倍放大倍数下,PBS对镜片表面蛋白的清除率为89.76％,2个不同厂家的隐形眼镜护理液对镜片表面蛋白的清除率分别为98.42％和98.27％.结论:扫描电镜观察并结合图像计算机统计分析法可对隐形眼镜护理液的蛋白清除效果进行评价.隐形眼镜护理液对镜片表面吸附的蛋白有显著清除效果.