基于RNA-Seq技术的向日葵油酸形成的转录组学分析

摘要

为探讨向日葵油酸调控机理,以高油酸材料J9和低油酸材料NK244为研究对象,在授粉后20d取材,采用RNA-Seq技术研究转录组.经比较分析,2个材料共鉴定到差异表达基因(DET)5 447个,其中上调表达基因2 709个,下调表达基因2 738个.对DET进行生物信息学分析,GO信息分析表明,有2 218个DET富集到57个功能条目,其中上调表达基因主要涉及催化活性、异构酶活性、碳水化合物结合、DNA结合、脂结合、碳水化合物过程、脂代谢过程和脂肪酸生物合成等;下调表达基因主要涉及蛋白激酶活性、ATP结合、蛋白磷酸化、tRNA氨酰化的蛋白质翻译和纤维素生物合成过程.KEGG分析中799个差异表达片段被显著富集在28个通路,主要为碳水化合物代谢通路和脂代谢通路.%Sunflower (Helianthus annuus L.) oil is considered a high quality edible oil, however the oleic acid formation mechanism have not been well characterized. In this study, two sunflower genotypes, previously classified as higher oleic acid concentration resource (J9) and lower oleic acid concentration resource (NK244), were used as research object. We used RNA-Seq to discuss and compare the transcriptome profile of the achene of 20 days after pollination. After the multiple comparisons, 5 447 DETs (differentially expressed genes) were observed in these two genotypes, including 2 709 up-regulated genes and 2 738 down-regulated genes. DETs were further researched by bioinformatics analysis, and 2 218 DETs were mapped into 3 GO categories, including 57 subcategories. Most of the up-regulated genes were demonstrated to participate in catalytic activity, heterogeneous enzymes, carbohydrates binding, DNA binding, lipid bing, carbohydrate process, lipid metabolism, fatty acid biosynthesis.Most of the up-regulated genes were demonstrated to involved in protein kinase activity, ATP binding, protein phosphorylation, tRNA ammonia acylation of protein translation, cellulose biosynthesis. KEGG analyses showed that 799 DEGs were classified into 28 pathways, and carbohydrate metabolism and lipid metabolism pathways included more DEGs. These finding could assist a better understanding of oleic acid formation mechanism of sunflower for quality breeding and germplasm innovation.