In order to research the biological functions of SGF14h and provide theoretical basis for studying the expression regulation of SGF14h,which was screened by previous mass spectrometry in our lab,the bioinformatic computer software,database and online programs were used to predict and analyze the nucleotide sequence and amino acid sequence of SGF14h.Results of gene structure analysis showed that SGF14h was located on the 4th soybean chromosome and its full length was 1 788 bp including 780 bp ORF which encoded 259 amino acids.The results of protein properties analysis showed that SGF14h sequence was rich in phosphorylation sites,including 10 serine phosphorylation sites and 3 threonine phosphorylation sites;SGF14h had no signal peptide and no transmembrane domain,so it belonged to non-secretion protein;it was found that SGF14h was located in mitochondria by subcellular localization prediction.The results of the protein structure analysis showed that there were 153 α-helix,0 β-sheet plus and 0 β-turn in SGF14h;SGF14h was a kind of hydrophilic protein and belonged to 14-3 -3 protein family.The results of phylogenetic tree analysis showed that SGF14h had the closest relationship with GF14 protein of maize.In dwarf soybean,SGF14 possibly played a key role in GA signal pathway and then affected the plant height.Therefore the study on SGF14h had biological significance,and these results could be the theoretical basis and provide evidences for further verifying the biological functions of SGF14h.%为了研究本实验室前期质谱分析筛选出的SGF14h蛋白的生物学功能,并为研究SGF14h基因的表达调控提供理论依据,使用一系列生物信息学软件、数据库和在线程序,对大豆SGF14h蛋白序列及核酸序列进行分析和预测.SGF14h基因结构分析显示:该基因位于第4号染色体上,全长1 788bp,其中开放阅读框长780bp,编码259个氨基酸.SGF14h蛋白属性分析显示:序列中富含磷酸化位点(丝氨酸磷酸化位点10个和苏氨酸磷酸化位点3个);此蛋白为非分泌型蛋白、无跨膜结构域;预测其定位于线粒体.SGF14h蛋白结构分析显示:二级结构有153个α螺旋,无β折叠和β转角;为亲水性蛋白质;属于14-3-3蛋白家族成员.SGF14h蛋白进化树分析表明:该蛋白与玉米GF14蛋白亲缘性最近.矮秆大豆中,SGF14h可能在GA信号转导中起重要作用,进而影响株高.因此对SGF14h的研究具有生物学意义,此结果可为进一步验证大豆SGF14h蛋白的生物学功能提供借鉴和理论依据.
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