Inhibitory activities of microalgal fucoxanthin against α-amylase, α-glucosidase, and glucose oxidase in 3T3-L1 cells linked to type 2 diabetes

摘要

Postprandial hyperglycemia is an early indication of type 2 diabetes and the target of many anti-diabetic and anti-obesity studies. α-Glucosidase and α-amylase are the crucial factors in regulating starch digestion and glucose absorption, making them key targets for many studies to treat postprandial hyperglycemia. We studied the inhibitory activities of microalgal fucoxanthin against rat-intestinalα-glucosidase and pancreatic α-amylase along with the antidiabetic effect to induce differentiation in 3T3-L1 pre-adipocytes using Oil Red-O staining. Fucoxanthin displayed strong hindrance activities towardα-amylase in a concentration-dependent manner, with an IC50 value of 0.68 mmol/L, whereas weak inhibitory activity against α-glucosidase, with an IC50 value of 4.75 mmol/L. Fucoxanthin also considerably elevated glucose oxidase activity in 3T3-L1 cells by 31.3％ at 5 μmol/L. During adipocyte differentiation, fucoxanthin showed lipid accumulation in 3T3-L1 cells with no cytotoxicity up to 20 μmol/L. However, fucoxanthin had no inhibitory activity on glucose-6-phosphate dehydrogenase. These results suggest that fucoxanthin might be useful for the prevention of obesity or diabetes by inhibiting carbohydrate-hydrolyzing enzymes and lipid accumulation and be utilized as an ingredient for a functional food or dietary supplement.