Objective To investigate whether or not recurrent subconvulsive epileptiform discharges may facilitate the persistent emotionality disorder in rats. Methods Sixty-two male SD rats aged from 6 to 7 weeks old were selected and divided randomly into 4 groups: SED group (n = 16)for rats with recurrent subconvulsive epileptiform discharges, HK group (n = 16) for hippocampal kindling, EC group (n = 15) for control of electrode, NC group(n =15) for normal control. The changes in emotionality of experimental rats were tested by locomotor activity, exploratory behavior, capture-resistance and elevated plus-maze(EPM) test at 1,7,30 d after electrical stimulations. Results Compared with the EC group, at 7 d after electrical stimulation,rats in the HK,SED group displayed a significantly decrease in number of creeping(P < 0.01), and the standing on hind limbs, the percentages of open-arm entries and open-arm retention time in EPM test was decresed (P < 0.05), and the difference still existed at 30 day after electrical stimulation (P < 0.05). And the capture-resistance in rats in the HK, SED group was significantly increased at 30 day after electrical stimulation (P < 0.01). Conclusions Recurrent subconvulsive epileptiform discharges causes the persistent emotionality as decreased locomotor activities, suppressed exploratory behavior, enhanced startle, delayed habituation, increased anxiety and defense reaction.%目的 探讨反复惊厥阈下痫样放电是否可引发实验大鼠较长时间的情绪唤醒障碍.方法 选择6～7周龄雄性SD大鼠62只,成组设计,随机分为惊厥阈下痫样放电组(subelinical epileptifor mdis charges group,SED组,n=16)、海马快速电点燃组(hippoeampal kindling group,HK组,n=16)、海马电极埋植对照组(control of electrode group,EC组,n=15)、正常对照组(normal control group,NC组,n=15),建立大鼠反复SED动物模型,通过运动活性、探究行为、拒俘反应性、高架十字迷宫实验,观测电刺激停止后1、7和30 d时实验大鼠情绪唤醒水平改变.结果 与EC组相比,电刺激后7 d,HK组、SED组大鼠旷场爬越行为明显减少(P<0.01),后肢性站立、进入高架十字迷宫开臂次数百分比和滞留时间百分比降低(P<0.05),电刺激后30 d上述差异仍存在(P<0.05),同时,HK组、SED组大鼠拒俘反应性在电刺激后30 d有明显增加(P<0.01).结论 反复惊厥阈下痫样放电引起了实验大鼠持续性运动活性减少、探究行为受抑、警觉水平过高、焦虑不安状态、环境适应能力下降、惊恐逃避反应等多种情绪唤醒障碍.
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