早产大鼠在高氧暴露下肺组织高迁移率族盒蛋白4和β-连环素的表达及意义

摘要

Objective To study the expression of high mobility group box protein 4 (SOX4) and β-catenin in lung tissues of preterm rats with hyperoxia exposure,and to study its significance.Method One-day-old preterm Sprague Dawley rats were randomly assigned into 4 groups:95％ hyperoxia group,70％ hyperoxia group,45％ hyperoxia group and air group.After three days,pathological changes of the lung tissues were observed by hematoxylin eosin staining,the mRNA expressions of SOX4 and β-catenin in lung tissues were detected by semi-quantitative reverse transcription polymerase chain reaction,and the protein expressions of SOX4 and β-catenin in the lung tissues were measured by western blot.Result The structure of lung tissues in air group was normal.45％ hyperoxia group represented mainly effusion and inflammatory cell infiltration,70％ and 95％ hyperoxia group showed acute lung injury characterized by inflammatory cell infiltration,hyperaemia,hemorrhagic change,disorganization and collapse of alveolar.The expressions of SOX4 mRNA and protein were highly elevated in all hyperoxia groups than that in air group (P ＜ 0.01);Compared with 45％ hyperoxia group,the expressions of SOX4 mRNA and protein were higher in 95％ hyperoxia group (P ＜ 0.01).β-catenin mRNA,total β-catenin protein and nuclear β-catenin protein were also highly elevated in hyperoxia groups compared with air group (P ＜ 0.05).Compared with 45％ hyperoxia group,the expressions of β-catenin mRNA and total β-catenin protein were also highly raised in 70％ hyperoxia group and 95％ hyperoxia group (P ＜ 0.05).The expression of nuclear β-catenin protein was higher in 95％ hyperoxia group than that in 45％ hyperoxia group (P ＜ 0.01).Conclusion The mRNA and protein expressions of SOX4 and β-catenin in lung tissues of preterm rats were increased by hyperoxia exposure.This mechanism may take part in hyperoxia-induced preterm rats lung injury.%目的 探讨早产大鼠在高氧暴露下肺组织高迁移率族盒蛋白4(high mobility group box protein 4,SOX4)和β-连环素(β-catenin)的表达及意义.方法 选择1日龄早产Sprague Dawley大鼠48只,随机分为95％高氧组、70％高氧组、45％高氧组和空气对照组,每组各12只.于生后第3天取肺组织,采用逆转录-聚合酶链反应及蛋白质免疫印迹技术检测各组肺组织SOX4和β-catenin mRNA及蛋白表达,并行病理检查.结果 空气对照组早产大鼠肺组织形态结构基本正常,间质炎症细胞浸润少;45％高氧组肺组织出现明显渗出和炎症细胞浸润;70％和95％高氧组肺组织可见明显炎症细胞浸润、充血、出血性改变及肺泡结构紊乱、肺泡压缩.各高氧组SOX4、β-catenin mRNA及SOX4、总β-catenin蛋白表达均高于空气对照组,且95％和70％高氧组高于45％高氧组[SOX4 mRNA:(0.81±0.09)、(0.66±0.06)比(0.29±0.09),β-catenin mRNA:(1.21±0.30)、(1.05±0.29)比(0.80±0.18),SOX4蛋白:(1.12±0.21)、(0.87±0.04)比(0.63±0.04),总β-catenin蛋白:(1.57±0.13)、(1.26 ±0.09)比(0.75 ±0.06)];各高氧组胞核β-catenin蛋白高于空气对照组,且95％高氧组高于45％高氧组[(1.21±0.21)比(0.65±0.16)],差异均有统计学意义(P＜0.05).结论 早产大鼠肺组织SOX4和β-catenin受高氧诱导表达增加,可能参与了早产大鼠高氧肺损伤.