骨形成蛋白4在人脑胶质瘤中的表达及临床意义

摘要

目的 探索骨形成蛋白4 (BMP4)在人脑胶质瘤中的表达,与临床特征间的关系及其在临床预后判断中的作用.方法 收集中国脑胶质瘤基因组图谱计划(CGGA)数据库中305例脑胶质瘤病人的临床资料和mRNA芯片结果,进行BMP4表达分析,并在REMBRANDT、GSE16011、TCGA数据库中进行验证.Kaplan-Meier生存曲线及Cox分析评价BMP4在预后判断中的作用,基因本体分析(GO)进一步分析BMP4正相关表达基因的功能.结果 BMP4表达水平随胶质瘤病理级别升高而降低.BMP4表达与病人KPS评分、异柠檬酸脱氢酶1(IDH1)基因突变情况密切相关.BMP4低表达者预后生存期短.BMP4可能与胶质细胞、星形细胞的发育、分化有关.结论 BMP4在胶质瘤发生、发展中可能起抑癌基因作用,可作为预后判断指标及潜在个体化治疗靶点.%Objective To investigate the expression of bone morphogenetic protein 4 (BMP4) in glioma,its relationship to clinical features and role in judging clinical prognosis.Methods Clinical data and mRNA microchip data of 305 glioma samples were obtained from Chinese Glioma Genome Atlas (CGGA) database,and BMP4 expression was analyzed,and which was validated by REMBRANDT,GSE16011 and TCGA datasets.Kaplan-Meier survival and Cox analysis were used to estimate the role of BMP4 in judgement of prognosis.Moreover,Gene ontology (GO) was performed for analyzing the gene fanction of BMP4 positive correlation expression.Results BMP4 expression level gradually decreased with increase of pathological grade in glioma.BMP4 expression was associated with patient's Kamofsky score and isocitrate dehydrogenase 1 (IDH1) mutation status.Furthermore,low BMP4 expression conferred a worse survival.Additionally,GO analysis showed that BMP4 was associated with development and differentiation of glial and astrocyte cells.Conclusion BMP4 might be a tumor suppressor and also serves as a novel judgement indicator for prognosis with potential therapeutic implications in glioma.