目的 研究密度感应分子(quorum sensing molecule)tyrosol(对羟基苯乙醇)和farnesol(法呢醇)对白念珠菌生物被膜形成的调控作用.方法 在tyrosol和farnesol干预下构建白念珠菌临床株和标准株生物被膜,在倒置显微镜下观察细胞形态,应用RT-PCR技术检测密度感应分子对白念珠菌HTA1和EFG1基因表达的调控作用,并采用MTT法观察密度感应分子对细胞活性的影响.结果 tyrosol对白念珠菌生物被膜的菌丝发生和细胞活性无明显促进作用,也无法中和farnesol对菌丝发生和细胞活性的抑制作用.tyrosol使白念珠菌生物被膜内细胞HTA1的表达增强,对EFG1的表达并无明显影响;tyrosol不能改变famesol对HTA1和EFG1表达的抑制作用.结论 tyrosol能在一定程度恢复口腔白念珠菌生物被膜内细胞的活跃状态,但当tyrosol与famesol同时存在时,tyrosol的作用被后者的抑制效应所掩盖,细胞对farnesol更敏感.%Objective To study the regulation of quorum sensing molecule tyrosol and farnesol on biofilm formation of Candida albicans. Methods Candida albicans biofilms of clinic isolates and standard strain SC5314 were built when quorum sensing molecule existed. And inverted microscope was used to observe the morphology of C. albicans cells. RT-PCR and MTT assay were carried out to investigate the effect of quorum sensing molecule on expression of the two genes (HTA1 and EFG1) and cytoactive. Results Tyrosol could not promote hyphae development and cytoactive of C. albicans biofilms. The expression of HTA1 of C. albicans in biofilms was up-regulated by tyrosol but EFG1 was not. The inhibitory effect of farnesol on hyphae development, cytoactive and gene expression were not changed by addition of tyrosol. Conclusion Tyrosol can make C. albicans biofilms active in early stage. But when tyrosol and farnesol were simultaneously added, the effect of tyrosol were masked by farnesol. And C. albicans cells were more sensitive to farnesol than to tyrosol.
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