目的 探讨以99mTc标记Gd-DTPA-BMA的可行性及其在荷瘤裸鼠体内分布的特征.方法 采用氯化亚锡还原法,用99mTc标记Gd-DTPA-BMA,TLC法测定99mTc-Gd-DTPA-BMA的放射化学纯度,将标记物注入荷A549肿瘤裸鼠体内,分别行SPECT显像及MRI增强成像,并测定其体内分布.结果 99mTc-Gd-DTPA-BMA放射化学纯度为99.2%,室温放置6 h后放射化学纯度大于95%.该标记物主要通过肾脏排泄,注射后肿瘤组织在SPECT及MRI下均能获得较好的显示或增强,且在肿瘤组织中具有较高的每克组织百分注射剂量率.结论 作为一种潜在的双模式显像剂,99mTc-Gd-DTPA-BMA值得进一步研究.%Objective To explore the feasibility of using 99mTc labeled Gd-DTPA-BMA, and to observe its characteristics of distribution in nude mice bearing tumor. Methods The labeled compounds were prepared by reducing 99mTc in the presence of Gd-DTPA-BMA with stannous chloride (SnCl2). The radiochemical purity was determined by TLC. 99mTc-Gd-DTPA-BMA was injected into nude mice bearing A549 tumor. SPECT and MR imaging were performed, respectively. The in vivo bio-distribution in nude mice was observed. Results The radiochemical purity of 99mTc-Gd-DTPA-BMA was 99.2％,and remained more than 95 ％ within 6 h at room temperature. 99mTc-Gd-DTPA-BMA imaging and bio-distribution experiments showed that the marker was excreted by renal. After inject 99mTc-Gd-DTPA-BMA, SPECT and MRI showed the tumor tissue or enhancement clearly, and high percentage of the injected dose per gram of tissues mass was noticed. Conclusion As a potential dual-modal imaging agent, 99mTc-Gd-DTPA-BMA is worthy of further research.
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